HEPES - Wikipedia
网页HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid) is a zwitterionic sulfonic acid buffering agent. It is one of the twenty Good's buffers.
HEPES Buffer (1 M, 7.5 pH) Preparation and Recipe
网页Learn how to prepare 1 L of HEPES buffer with a pH of 7.5 for cell culture medium. Find the required components, concentrations, and adjustment steps in this online tool.
Universal buffers for use in biochemistry and biophysical …
网页Individual curves for HEPES, MES, and NaAc show buffering range of specific weak acids. HEPES is a diprotic acid and has two relevant pKa’s. When combined into a universal buffer, a linear response to the addition of acid is observed across the entire tested range.
HEPES (1M) - Thermo Fisher Scientific
网页Gibco HEPES (1M) is a zwitterionic organic chemical buffering agent used in cell culture media. It is membrane impermeable, stable, and has low light absorbance. It is available in different quantities and prices online.
HEPES Buffer - MilliporeSigma
网页HEPES is a zwitterionic organic compound that acts as a buffer for maintaining physiological pH levels in biochemical research and cell culture. Find out the different grades, forms and uses of HEPES buffer from Sigma-Aldrich, a …
网页Learn about the principles and applications of buffers in biological research, including pH, pKa, buffer capacity, and buffer media. This booklet from EMD Biosciences provides useful information and tips for choosing and preparing buffers for your experiments.
Biological pH buffers in IVF: help or hindrance to success
网页2011年5月26日 · HEPES (4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid ) (HEPES) is a zwitterionic organic buffer, also known as a Good’s buffer, that is a modified taurine molecule. HEPES can be utilized as a free acid, or conjugated to various salts (Fig. 2).
4- (2-Hydroxyethyl)-1-piperazine ethanesulfonic acid - PubChem
网页2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid is a HEPES that is ethanesulfonic acid in which one of the methyl hydrogens is replaced by a 4-(2-hydroxyethyl)piperazin-1-yl group. A Good's buffer substance, pKa = 7.55 at 20 ℃.
HEPES buffer - CSH Protocols
网页HEPES: 4.77 g: 20 mM: H 2 O to 1 L: Adjust the pH to 7.4 with HCl or NaOH. HEPES buffer can be stored refrigerated for several weeks.
网页1996年9月17日 · HEPES is a zwitterionic buffer for biological research, tissue culture and protein applications. Learn about its physical and chemical properties, solubility, usage, preparation and quality control.