Ever since recombinant DNA has been used to develop and manufacture vaccines, antivaxxers have portrayed it as evil. This ...
To improve traceability and enable authentication of synthetic nucleic acid sequences, researchers are embedding digital ...
The AAV viral vectors, as well as messenger RNAs derived from plasmid DNA templates ... While real-time PCR is still the gold standard for quantifying therapeutic viral vectors like AAV used ...
Things haven’t got much better over the years, largely shutting STEM classes and biohackers out of the PCR market. That may be about to change, though, if the €99.00 PocketPCR thermal cycler ...
In recent years, quantitative real-time PCR tests have been extensively developed in clinical microbiology laboratories for routine diagnosis of infectious diseases, particularly bacterial diseases.
After vortexing, the plasmid was pelleted in a microcentrifuge and washed with 800 μl 50% isopropanol solution of 10 mM MgCl 2, 300 mM NaCl, and 25 mM EDTA. The final product was PCR-sequenced ...
HLA typing can be performed using numerous methods, including endpoint PCR, qPCR, Sanger sequencing, and next-generation ...
The following table lists the recommended amount of DNA template and primer for optimal Sanger sequencing results. Note: for plasmid DNA the size is the entire plasmid, vector plus insert. For plasmid ...
Global Plasmid Purification Market was valued at approximately USD 1.78 billion in 2023, exhibiting a robust CAGR of 11.69% throughout the forecast period from 2024 to 2032. Read the Market Summary ...
Vector production (clinical-grade plasmid DNA and retroviral vectors ... and transgene expression in patient cells and tissues (FACS analysis, real-time PCR, immunostaining, etc.). Quality control and ...
The work presented will be of interest to cancer biologists and more broadly to those interested in NSCLC translational studies. Non-small cell lung cancer (NSCLC) is the most common subtype of lung ...