The detailed protocol is described in Extended methods (Appendix 1). The eluted mitochondria were used in malachite green assay for Pi estimation, boiled with SDS-glycerol buffer for western blots or ...
This valuable paper seeks to determine the role of endogenous CNS hemoglobin in protecting mitochondrial homeostasis in hypoxia. There is merit in the work, although it remains incomplete as there is ...
Figure 4. The Cell Painting assay modified for 3D spheroids. Spheroids were labeled with phalloidin, MitoTracker, WGA, SYTO 14, concanavalin A and Hoechst 33342. Shown here is an example image of ...